built-in inside the bottom of Cellbag), the concentration of O2 and

CO2 in the inlet gas mixture, as well as the total gas flow rate. The

range of operating parameters that can be applied in system of

WAVE 25 has been presented in Table 1.

Fig. 2 Schematic diagram of Cellbag container with arrangement of inlet and

outlet ports: (1) Cellbag rods, (2, 6, 7) various pipe-ports, (3) outlet vent filter with

pressure control valve, (4) inlet vent filter, (5) Screwcap port, (8) CLAVE™

sampling port

Table 1

The range of operating parameters that can be applied in WAVE 25 system

Operating parameters

Range of parameter

Unit

Angle of oscillations

2–12



Frequency of oscillations

2–40

min
¹

Gas flow rate

0.1–1.0

L min
¹

Volume of liquid phase

0.1–1.0

L

Temperature

20–40

C

O2 concentration in gas phase

0–50

%

CO2 concentration in gas phase

0–15

%

Rocking motion acceleration

15–100

%

150

Kamil Wierzchowski and Maciej Pilarek